Replication of Salmonella enterica Serovar Typhimurium in Human Monocyte-Derived Macrophages

Supplemental material

• Supplemental file 1 -

  Fig. S1. Phenotype of human monocyte-derived macrophages differentiated with MCSF alone (M0) or with addition of IFN-γ and LPS (M1) or IL-4 (M2). Fig. S2. Infection of macrophages with SPI1-expressing bacteria does not induce pyroptosis at a low MOI. Fig. S3. Comparison of cells differentiated with fetal bovine serum (FBS) or human serum (HuS). Legends to Movies S1 and S2.

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• Supplemental file 2 -

  Movie S1. Live cell microscopy of infection by WT Salmonella.

  MOV, 7.8M

• Supplemental file 3 -

  Movie S2. Live cell microscopy of infection by ΔSPI2 Salmonella.

  MOV, 5.3M