Enterococcus faecalis Gelatinase Mediates Intestinal Permeability via Protease-Activated Receptor 2

Supplemental material

• Supplemental file 1 -

  Legends to Fig. S1 to S5.

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• Supplemental file 2 -

  Fig. S1. Caco-2 and T-84 monolayers were incubated with CCM from E. faecalis V583.

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• Supplemental file 3 -

  Fig. S2. T-84 monolayers were incubated with CCM from E. faecalis V583 and E. faecalis ΔgelE.

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• Supplemental file 4 -

  Fig. S3. (A) E. faecalis V583 mediates monolayer permeability of Caco-2 via PAR2. (B) T-84 monolayers were incubated basally with or without FSLLRY-NH2 for 24 h. (C) E. faecalis V583 CCM-mediated permeability of T-84 monolayers was not reduced when PAR2 activation was blocked with an antagonist; however, the TEER of the monolayers were impacted.

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• Supplemental file 5 -

  Fig. S4. The permeability of the mouse colonic epithelium is not different between WT and PAR2^-/- mice.

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• Supplemental file 6 -

  Fig. S5. E. faecalis V583 CCM impacts the detection of PAR2 in epithelial cells over time.

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