Published July 6, 2015 // JCB vol. 210 no. 1 79-97
The Rockefeller University Press, doi: 10.1083/jcb.201503117

Interphase centrosome organization by the PLP-Cnn scaffold is required for centrosome function

Files in this Data Supplement:

  • Supplemental Material (PDF)
  • Video 3 -
    PLPFL in a cycling plp− embryo. Live plp−Drosophila embryo expressing PLPFL-GFP (white). Images were analyzed by time-lapse spinning disk confocal microscopy (Ti Eclipse; Nikon). Frames were taken every 30 s for 20.5 min.
  • Video 4 -
    PLP satellite dynamics. Live plp−Drosophila embryo expressing PLPFL-GFP (white). Images were analyzed by time-lapse spinning disk confocal microscopy (Ti Eclipse; Nikon). Frames were taken every 500 ms for 30 s.
  • Video 10 -
    PLPΔ5 in a cycling plp− embryo. Live plp−Drosophila embryo expressing PLPΔ5-GFP (white). Images were analyzed by time-lapse spinning disk confocal microscopy (Ti Eclipse; Nikon). Frames were taken every 30 s for 18.5 min.
  • Video 2 -
    Cnn-GFP during the mitosis-to-interphase transition in a control embryo. Live control Drosophila embryo expressing Cnn-GFP (white). Images were analyzed by time-lapse spinning disk confocal microscopy (Ti Eclipse; Nikon). Frames were taken every 300 ms for 5 min.
  • Video 6 -
    Cnn-mCherry in a control and plp− embryo. Live control and plp−Drosophila embryos expressing Cnn-mCherry (white). Images were analyzed by time-lapse spinning disk confocal microscopy (Ti Eclipse; Nikon). Frames were taken every 500 ms for 30 s.
  • Video 8 -
    GFP-MT in a control and plp− embryo. Live control and plp−Drosophila embryos expressing GFP-MT (white). Images were analyzed by time-lapse spinning disk confocal microscopy (Ti Eclipse; Nikon). Frames were taken every 30 s for 15 min.
  • Video 1 -
    Cnn-GFP and H2A-RFP through multiple NCs in a control embryo. Live control Drosophila embryo expressing Cnn-GFP (green) and H2A-RFP (red). Images were analyzed by time-lapse spinning disk confocal microscopy (Ti Eclipse; Nikon). Frames were captured every 10 s for 26.5 min.
  • Video 5 -
    PLPFL and Cnn-mCherry are packaged as dynamic particles. Live control Drosophila embryo expressing PLPFL-GFP (white) and Cnn-mCherry (red). Images were analyzed by time-lapse spinning disk confocal microscopy (Ti Eclipse; Nikon). Frames were taken every 1 s for 30 s.
  • Video 9 -
    H2A-RFP in a control and plp− embryo. Live control and plp−Drosophila embryos expressing H2A-RFP (white). Images were analyzed by time-lapse spinning disk confocal microscopy (Ti Eclipse; Nikon). Frames were taken every 30 s for 15 min for WT and 17.5 min for the mutant.
  • Video 7 -
    GFP-γTub through multiple NCs in a control and plp− embryo. Live control and plp−Drosophila embryos expressing GFP-γTub (white). Images were analyzed by time-lapse spinning disk confocal microscopy (Ti Eclipse; Nikon). Frames were taken every 30 s for 21 min for WT and 27 min for the mutant.