Supplemental Data
Files in this Data Supplement:
- Supplemental video S1 (.mov, 68 KB) - Supplemental video S1: Representative video of kinesin-1 motile events on microtubules in the presence of untranfected control COS7 lysates. Untransfected control lysates rarely produce microtubule binding or motile events, and microtubule binding events are short-lived when they do occur. (Supplemental videos S1-4) First frame shows rhodamine-labeled microtubules, all subsequent frames show mCit-positive KLC puncta. 100 frames of a 1 FPS video are shown here at a 7 FPS rate (7x speed). Arrows indicate start points of motile events, arrowheads indicate nonmotile events. Scale bar 5 ?m.
- Supplemental video S2 (.mov, 23 KB) - Supplemental video S2: Representative video of kinesin-1 motile events on microtubules in the presence of WT JIP3 COS7 lysates. Binding events are long-lived and often result in motility. Motile events are frequently robust, fast and long-lived. Arrows indicate start points of motile events, arrowheads indicate nonmotile events. Scale bar 5 ?m.
- Supplemental video S3 (.mov, 496 KB) - Supplemental video S3: Representative video of kinesin-1 motile events on microtubules in the presence of JIP3ΔKLC COS7 lysates. Binding events are infrequent but often result in motile events. Motile events are not as long-lived as those produced in the presence of WT lysates, but are relatively long-lived and fast compared to untransfected controls. Arrows indicate start points of motile events, arrowheads indicate nonmotile events. Scale bar 5 ?m.
- Supplemental video S4 (.mov, 78 KB) - Supplemental video S4: Representative video of kinesin-1 motile events on microtubules in the presence of JIP3ΔKHC COS7 lysates. Binding events are frequent but rarely result in motility. Motile events are short and slow relative to those produced in the presence of WT JIP3 lysates. This video shows a looped bundle of two microtubules, hence the motility of the highlighted event is bidirectional. As non-motile events are abundant under these conditions, only some are highlighted in this video. Arrows indicate start points of motile events, arrowheads indicate non-motile events. Scale bar 5 ?m