Analysis of NbaY-LBD by intrinsic tryptophan fluorescence and CD spectroscopy (Fig. S1), growth curves (Fig. S2), genetic complementation of P. putida KT2440R ΔmcpP using quantitative capillary chemotaxis assays (Fig. S3), conservation of amino acids involved in pyruvate recognition of CACHE sensor domains (Fig. S4), NbaY-LBD homology model (Fig. S5), and compounds used for microcalorimetric binding studies (Table S1).
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