Supplemental materials and methods (detection of AOH under various growth conditions; phytotoxicity, antigerminative and antibacterial activity assays); primers used in this study (Table S1); KS domain sequences included in the phylogenetic analysis (Table S2); detection of AOH under different growth conditions by LC-QToF/MS (Fig. S1); gene expression profile of the P. nodorum SnPKS19 gene cluster during growth in planta and in vitro (Fig. S2); SnPKS19 knockout scheme and diagnostic PCR for confirming SnPKS19 deletion mutants (Fig. S3); Southern blot confirmation of the SnPKS19 knockout (Fig. S4); comparison of the gene structure of the revised SnPKS19 (SNOG_15829) with the original annotation (Fig. S5); reverse transcriptase PCR of SnPKS19, pkgA, and pkgB (Fig. S6); wheat detached leaf assay of P. nodorum strains and phytotoxicity assay of AOH (Fig. S7).
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