The Rate and Molecular Spectrum of Spontaneous Mutations in the GC-Rich Multichromosome Genome of Burkholderia cenocepacia
Supporting Information
Supporting Information
- Supporting Information - Figures S1-S8, File S1, and References (PDF, 207 KB)
- Figure S1 - Estimates of the number of generations each line experienced per day, measured monthly over the course of the MA experiment. (PDF, 164 KB)
- Figure S2 - Frequency distributions of the number of base substitutions (A) and indels (B) identified per line. (PDF, 151 KB)
- Figure S3 - Distribution of the full-coverage read frequencies leading to the identification of each insertion-deletion mutation (indel) in this study. (PDF, 132 KB)
- Figure S4 - Distribution of the number of MA lines that identified each insertion-deletion mutation (location, size, and motif) called in this study. (PDF, 132 KB)
- Figure S5 - Chromosome size and expression (derived from RNAseq as described in (Gout et al. 2013)) decline from chromosome 1 (chr1) to chromosome 3 (chr3) (A,B), while evolutionary rate is lowest on chr1 and highest on chr3 (C) (Morrow and Cooper 2012). (PDF, 138 KB)
- Figure S6 - Relationship between base substitution mutation rate per effective genome size per generation and effective population size (NE) in five multicellular eukaryotes (red), seven unicellular eukaryotes (black), and eight prokaryotes (blue; B. cenocepacia - green) (Sung et al. 2012). (PDF, 133 KB)
- Figure S7 - Relative conditional substitution rates at seven B. cenocepacia loci (atpD, gltB, gyrB, lepA, phaC, recA, and trpB). (PDF, 133 KB)
- Figure S8 - The ratio of coding (Black) to non-coding (Grey) substitutions for each B. cenocepacia substitution type. (PDF, 132 KB)
- File S1 - Supplementary Tables S1 and S2. (.xlsx, 56 KB)