Evidence That the Origin of Naked Kernels During Maize Domestication Was Caused by a Single Amino Acid Substitution in tga1

Files in this data supplement:

  • Supporting Information: File S1, Figures S1-S5, and Table S1 (PDF, 910 KB)
  • File S1: Material and Methods (PDF, 182 KB)
  • Figure S1: Nucleotide sequence alignment (polymorphic sites only) of the 1042-bp region of tga1 identified as containing the causative site (Wang et al. 2005). (PDF, 655 KB)
  • Figure S2: Amino acid alignment of TGA1 and NOT1 from the maize inbred W22 and the teosinte parent of the mapping population used by Wang et al. (2005). (PDF, 1 MB)
  • Figure S3: Column graph showing results of RT-qPCR using not1 specific primers. (PDF, 123 KB)
  • Figure S4: Nucleotide sequences of the maize and teosinte not1 promoter sequences. (PDF, 725 KB)
  • Figure S5: Western blot showing the TGA1 and NOT1 protein accumulation were knocked down or out in 4 different tga1-RNAi events. (PDF, 160 KB)
  • Table S1: List of additional teosinte accessions for diversity analysis. (PDF, 118 KB)