Lrrk regulates the dynamic profile of dendritic Golgi outposts through the golgin Lava lamp

Files in this Data Supplement:

• Supplemental Material (PDF)
• Video 4 -
  GOP with YFP-Lrrk colocalization moves in anterograde direction. Transgenes for expression of ManII-GFP (green) and YFP-Lrrk (red) were driven by ppk-GAL4 in da neurons and imaged by laser-scanning confocal microscopy (LSM710; Carl Zeiss) in 20-s intervals during an 8-min period. One dynamic GOP (arrow) labeled by both ManII-GFP and YFP-Lrrk moves in the dendrite and reaches a more distal position. Distal to the left and proximal to the right. Bar, 2 µm.
• Video 3 -
  GOP free of YFP-Lrrk colocalization moves in retrograde direction. Transgenes for expression of ManII-GFP (green) and YFP-Lrrk (red) were driven by ppk-GAL4 in da neurons and imaged by laser-scanning confocal microscopy (LSM710; Carl Zeiss) in 20-s intervals during an 8-min period. One moving GOP (arrow) labeled only by ManII-GFP free of YFP-Lrrk moves bidirectionally and reaches a more proximal position in the dendrite. Distal to the left and proximal to the right. Bar, 2 µm.
• Video 5 -
  GOP with YFP-Lrrk colocalization moves in retrograde direction. Transgenes for expression of ManII-GFP (green) and YFP-Lrrk (red) were driven by ppk-GAL4 in da neurons and imaged by laser-scanning confocal microscopy (LSM710; Carl Zeiss) in 20-s intervals during an 8-min period. One dynamic GOP (arrow) labeled by both ManII-GFP and YFP-Lrrk moves in the dendrite and reaches a more proximal position. Distal to the left and proximal to the right. Bar, 2 µm.
• Video 1 -
  GOP with colocalized YFP-Lrrk is stationary in dendrites. Transgenes for expression of ManII-GFP (green) and YFP-Lrrk (red) were driven by ppk-GAL4 in da neurons and imaged by laser-scanning confocal microscopy (LSM710; Carl Zeiss) in 20-s intervals during an 8-min period. One stationary GOP (arrow) labeled by both ManII-GFP and YFP-Lrrk stalls in the dendrite. Distal to the left and proximal to the right. Bar, 2 µm.
• Video 2 -
  GOP free of YFP-Lrrk colocalization moves in anterograde direction. Transgenes for expression of ManII-GFP (green) and YFP-Lrrk (red) were driven by ppk-GAL4 in da neurons and imaged by laser-scanning confocal microscopy (LSM710; Carl Zeiss) in 20-s intervals during an 8-min period. One moving GOP (arrow) labeled only with ManII-GFP free of YFP-Lrrk moves bidirectionally and reaches a more distal position in the dendrite. Distal to the left and proximal to the right. Bar, 2 µm.