Phosphorylation of SAF-A/hnRNP-U Serine 59 by Polo-Like Kinase 1 Is Required for Mitosis

Supplemental material

• Supplemental file 1 -

  Fig. S1 (Expression of SAF-A in SAF-A WT and mutant cell lines), S2 (SAFA-S59 phosphorylation in mitosis), S3 (Endogenous SAF-A phosphorylation in nocodazole-treated cells), S4 (Interaction of SAF-A with PLK1), S5 (DNA-PK-dependent SAF-A S59 phosphorylation), S6 (Blocking peptide and SAF-A phosphor-S59 staining), S7 (Lack of signal for SAF-A pS59 in SAF‐A S59A cells), S8 (BI2536 and SAF-A S59 phosphorylation), S10 (SAF-A S59A cell cyclin B1 and securin expression in response to taxol), and S11 and S12 (PP6 [S11] and PP4c [S12] dephosphorylation of SAF-A S59) and Table S1 (Summary of data for FACS analysis)

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• Supplemental file 2 -

  Fig. S9A (Live-cell imaging of SAF-A WT cells)

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• Supplemental file 3 -

  Fig. S9B (Live-cell imaging of SAF-A S59A cells)

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• Supplemental file 4 -

  Fig. S9C (Live-cell imaging of SAF-A S59E cells)

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