Hierarchy of Carbon Source Utilization in Soil Bacteria: Hegemonic Preference for Benzoate in Complex Aromatic Compound Mixtures Degraded by Cupriavidus pinatubonensis Strain JMP134

Supplemental material

  • Supplemental file 1 -

    Growth and carbon source degradation curves of Cupriavidus pinatubonensis JMP134 grown in binary mixtures of aromatic compounds, and the corresponding carbon source removal, in the respective single-compound cultures (Fig. S1); growth substrate degradation time overlaps and degradation start times in C. pinatubonensis grown in binary mixtures (Fig. S2); catabolic routes of benzoate and 4-hydroxybenzoate (Fig. S3A), phenol (Fig. S3B), 3-hydroxybenzoate (Fig. S3C), 4-hydroxyphenylacetate and tyrosine through homogentisate (Fig. S3D), and phenylacetate (Fig. S3E) in C. pinatubonensis JMP134; beta-galactosidase levels obtained from transcriptional fusions of promoters of genes encoding PhlK1 and PhlK2 phenol hydroxylases in C. pinatubonensis cells previously grown on benzoate/phenol mixtures (Fig. S4); half-lives of aromatic compounds used as single compounds or in mixtures for growth of C. pinatubonensis JMP134 wild type or the ΔbenA mutant (Table S1); growth and degradation parameters of a continuously fed culture of C. pinatubonensis JMP134 with a six-member mixture of benzoate, 3- and 4-hydroxybenzoate, phenylacetate, tyrosine, and 2,4-dichlorophenoxyacetate (Table S2); some metabolic features of aromatic compounds used in this work (Table S3).

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