The Transcriptional Response to Raf Activation Is Almost Completely Dependent on Mitogen-activated Protein Kinase Kinase Activity and Shows a Major Autocrine Component
Mol. Biol. Cell Schulze et al.
15: 3450
Supplemental Material
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This article contains the following supporting material:
Supplemental Figures 1-2.pdf -
Supplemental figure 1. MCF-10A ΔRaf-ER and MCF-10A V12Ras cells were cultured in minimal medium either in the presence (4-OHT) or absence (control, V12Ras) of 100μM 4-OHT for 24 hours. Phosphorylation of p42ERK2/p44ERK1 was detected using a phosphospecific antibody. Supplemental figure 2. Activation of ΔRaf-ER in MCF-10A cells does not induce NF-κB dependent transcription MCF-10A ΔRaf-ER cells were transfected with 0.1μg of a NF-κB reporter construct (pBL3xNFkB-CAT) together with 0.5 μg of expression vectors for V12 Ras or empty vector, respectively. In parallel, cells were induced with 4-OHT for 24 or 48 hours. Data represent relative changes in CAT reporter activity and reflect the mean of three independent experiments.