Supporting Information

Files in this Data Supplement:

Fig. 6. The rhodamine-tagged hydroxamate benzophenone probe (HxBP-Rh) identifies dipeptidylpeptidase III (DPPIII) as a GM6001-sensitive metalloprotease present in the human breast cancer cell line MCF7. Shown is a representative HxBP-Rh labeling (100 nM) profile of the soluble proteome from MCF-7 cells. The labeling of an ≈80-kDa protein was blocked by GM6001 (5 mM), and this target was identified by using a trifunctional HxBP probe as DPPIII (single arrowhead; see Methods for more details on the isolation and identification of metalloproteases by using a trifunctional HxBP probe). Numbers on left of gel refer to protein molecular mass markers (kDa). Note that treatment with peptide N-glycosidase F (PNGaseF) does not alter the migration of DPPIII because this enzyme is a cytosolic, nonglycosylated protein.