Development of a Recombinant Escherichia coli Strain for Overproduction of the Plant Pigment Anthocyanin

Supplemental material

  • Supplemental file 1 -

    Cyanidin 3-­O-glucoside (C3G) concentration depicted in color intensities of different background strains harboring anthocyanin pathway enzymes in fusion construct and overexpression of UDP-glucose pathway enzymes (Fig. S1); C3G colorimetric assay development (Fig. S2); examples of 24-­ and 48-well plates used for fermentation parameter optimization (Fig. S3); HPLC chromatogram of cell culture before and after extraction with ethyl acetate (Fig. S4).

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