Use of Hybridization Chain Reaction-Fluorescent In Situ Hybridization To Track Gene Expression by Both Partners during Initiation of Symbiosis

Supplemental material

• Supplemental file 1 -

  Detailed HCR-FISH protocol, probe sequences (Table S1), tracking the position of bacteria during colonization (Fig. S1), and validation of the specificity of the luxA probe (Fig. S2).

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