Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie 1 - Movie S1. Drp1 preferentially remodels fluid-phase bilayers containing CL. Effect of addition of 0.5 μM (final) BODIPY-FL-labeled Drp1 WT (green) to GUVs phase-separated into lo (dark) and ld (Rh-DOPE-labeled; red) membrane regions. Preferential recruitment of BODIPY-FL-labeled Drp1 WT (green) to Rh-DOPE-labeled, 10 mol% native CL-containing ld regions (red) and the resultant membrane tubulation are seen. Movies were captured at ∼1 fps, and are played at ∼5 fps.
- Movie 3 - Movie S3. Effect of GMP-PCP on Drp1-mediated membrane constriction. Effect of addition of 0.5 μM (final) BODIPY-FL-labeled Drp1 WT (green) to Rh-DOPE-labeled, 25 mol% native CLcontaining GUVs (red) in the constant presence of GMP-PCP (1 mM). Movies were captured at ∼1 fps, and are played at ∼5 fps.
- Movie 2 - Movie S2. GTP-dependent constriction of CL-containing GUVs by Drp1. Effect of addition of 0.5 μM (final) BODIPY-FL-labeled Drp1 WT (green) to Rh-DOPE-labeled, 25 mol% native CLcontaining GUVs (red) in the constant presence of GTP (1 mM). Movies were captured at ∼1 fps, and are played at ∼5 fps.