Metabolic Impacts of Using Nitrogen and Copper-Regulated Promoters to Regulate Gene Expression in Neurospora crassa

Supporting Information for Ouyang et al., 2015

Files in this Data Supplement:

  • Supporting Information - Table S1 and Figures S1-S6 (PDF, 1 MB)
  • Table S1 - N. crassa metabolites detected by 1H NMR. (PDF, 74 KB)
  • Figure S1 - Regulation of the endogenous tcu-1 and nit-6 mRNAs. (PDF, 161 KB)
  • Figure S2 - Derepression of GFP mRNA and protein levels after exposure to different nitrate concentrations. (PDF, 199 KB)
  • Figure S3 - Expansion of the spectra of pnit-6_1.5 cultured on Gln (top) and nitrate (bottom) at lower vertical scale to highlight differences in the intensity the Ala resonance. (PDF, 135 KB)
  • Figure S4 - Overlays of the 1H NMR spectra for all six replicates of strain pnit-6_1.5. (PDF, 476 KB)
  • Figure S5 - Expansion of the spectra of wild-type N. crassa (WT) cultured on Gln (top) and nitrate (bottom). (PDF, 128 KB)
  • Figure S6 - Overlays of 1H NMR spectra for all five replicates of strain ptcu-1_1.5. (PDF, 471 KB)