Quantification of Azospirillum brasilense FP2 Bacteria in Wheat Roots by Strain-Specific Quantitative PCR

Supplemental material

  • Supplemental file 1 -

    Synteny plot of Azospirillum brasilense strains FP2 and Sp245 (Fig. S1); A. brasilense FP2 annotated genomic regions used for strain-specific primer pair design (Fig. S2); qPCR standard curve for A. brasilense FP2 (Fig. S3); qPCR comparison of the quantities of A. brasilense FP2 bacteria associated with wheat roots in the three inoculation experiments performed under sterile and nonsterile conditions without or with coinoculation of A. brasilense NH, Herbaspirillum seropedicae Z67, Gluconacetobacter diazotrophicus DSM 5601, or Azospirillum lipoferum DSM 1691 (Fig. S4).

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