Parasite Manipulation of the Invariant Chain and the Peptide Editor H2-DM Affects Major Histocompatibility Complex Class II Antigen Presentation during Toxoplasma gondii Infection

Supplemental material

  • Supplemental file 1 -

    Supplemental materials and methods. Supplemental results. Fig. S1. T. gondii upregulates CD74 protein expression in infected BMMΦ in the absence of IFN-γ until egress of the parasite. Fig. S2. CD74 does not colocalize with autophagosome or lysosome markers in infected unstimulated BMMΦ. Fig. S3. CD74 induction in infected BMMΦ occurs in the absence of endogenous production of TNF-α and type I IFNs. Fig. S4. In vitro replication rate of parasites in infected BMMΦ is not affected by the presence or absence of CD74. Table S1. PCR primers for mouse genotyping. Table S2. RT-PCR primers for class CD74 genes.

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