Supplementary Figure Legends

Supplementary Figure 1: Optimization of enriched dopaminergic neuronal culture. Three fields obtained from a culture without (A) or with (B) one-time addition of 10 ng/ml GDNF, 2 ng/ml TGF-b and 50 µM dcAMP, following a variation of the protocol described by Sun et al., 2004 in Cell Biology International 28:323-325. MAP2 (green), TH (red), nuclei (blue).

Supplementary Figure 2:  Time optimization for MPTP treatment. Fields of enriched dopaminergic neuronal culture at 24 h (A) and 48 h (B) when cells were treated with 100 µM MPTP in the presence or absence of 100 nM NPD1. b III tubulin (red), TH (green), nuclei (blue).

Supplementary Figure 3:  Time optimization for rotenone treatment. Fields of enriched dopaminergic neuronal culture at 24 h (A) and 48 h (B) when cells were treated with 100 nM rotenone in the presence or absence of 100 nM NPD1. bIII tubulin (red), TH (green), nuclei (blue).

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