The DYRK-family kinase Pom1 phosphorylates the F-BAR protein Cdc15 to prevent division at cell poles

Files in this Data Supplement:

• Supplemental Materials (PDF)
• Video 2 -
  Actomyosin ring formation in pom1^as1 mid1Δ. Time lapse of meGFP-Cdc15 (green) in pom1^as1 mid1Δ cells treated with 3MB-PP1 for 60 min before imaging. Images were taken every 4 min for 188 min and the movie is run at 3.6 frames per second. Note the inability of the actomyosin ring to slide toward the cell middle, with Cdc15 remaining attached to the cell poles. Note also the remarkable ability of the two strands of the ring originating from each cell pole to precisely meet. Bar, 5 µm.
• Video 1 -
  Actomyosin ring formation in mid1Δ. Time lapse of meGFP-Cdc15 (green) in mid1Δ cells with images taken every 2 min for 80 min. The video is run at 3.6 frames per second. Note the sliding of the actomyosin ring in two cells with the Cdc15-marked ring assembling at the cell tips before sliding toward the cell middle. Bar, 5 µm.