This PDF file includes:

• Fig. S1. Gallery of four cryo-EM maps obtained by classification.
• Fig. S2. Examples for map fittings.
• Fig. S3. Density for GTP in the nonrotated complex.
• Fig. S4. Interaction of protein S12 with domain III.
• Fig. S5. Densities linking domain III to IV and domain II to V in our maps.
• Fig. S6. Three-stage (I, II, III) classification of the entire data set using RELION.
• Fig. S7. Color code for the resolution values in the movie showing the local resolution map.
• Legends to movies S1 to S3

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.mov format). Overview and local resolution estimation for the 3.6-Å map.
• Movie S2 (.mov format). Examples of high-resolution protein features in the 3.6- Å map.
• Movie S3 (.mov format). Examples of high-resolution RNA features in the 3.6-Å map.