Scheme of the method to generate the interruption mutation in the gene coding for Nox of R. erythropolis strain MI2 by the thiostrepton resistance cassette in the BamHI restriction site (Fig. S1), strain growth (Fig. S2), utilization of DTDB by four Rhodococcus strains (Fig. S3), turbidity increases of cultures (Fig. S4), GC-MS analysis (Fig. S5), SDS-PAGE (Fig. S6), utilization capabilities of 49 carbon sources for both the wild type and transposon-induced mutants (Table S1), and genotypic characterizations (Table S2).
PDF, 481K