Metabolic Pathway Involved in 2-Methyl-6-Ethylaniline Degradation by Sphingobium sp. Strain MEA3-1 and Cloning of the Novel Flavin-Dependent Monooxygenase System meaBA

Supplemental material

  • Supplemental file 1 -

    Oligonucleotide primers used in gap closure (Table S1); results of the peptide mass of fingerprint analysis (Table S2); agarose gel electrophoresis of the ERIC-PCR patterns of strains MEA3-1 and MEA3-1Mut (Fig. S1); spontaneous ammonia release of the 4-OH-DMA in the water measured using the Nessler's reagent colorimetric method (Fig. S2); physical map of the circular plasmid pMEA02 from strain MEA3-1 (Fig. S3); representative 2-DE protein maps of strains MEA3-1 and MEA3-1Mut (Fig. S4); multiple sequence alignment of the MeaA amino acid sequence and related protein sequences (Fig. S5); effect of the different concentrations of the cytochrome P450 inhibitor metapyrone on the degradation of MEA (Fig. S6).

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