Complementation of Yeast Genes with Human Genes as an Experimental Platform for Functional Testing of Human Genetic Variants

Supporting Information for Hamza et al., 2015

Supporting Information

Supporting Information - Figures S1-S4 and Tables S1-S6 (PDF, 5 MB)
Figure S1 - Growth curve replicates of wild-type yeast (BY4742) expressing indicated human cDNAs in -Leu media at 30°C. (PDF, 1 MB)
Figure S2 - Growth curve replicates of cdc9∆ yeast strains expressing indicated human cDNAs in -Leu media at 30°C. (PDF, 1 MB)
Figure S3 - Growth curve replicates of pob3∆ yeast strains expressing indicated human cDNAs in -Leu media at 30°C. (PDF, 1 MB)
Figure S4 - Growth curve replicates of glc7∆ yeast strains expressing indicated human cDNAs in -Leu media at 30°C. (PDF, 1 MB)
Table S1 - Plasmids and strains used for screening tumor-speciﬁc variants in yeast. (.xlsx, 12 KB)
Table S2 - Essential yeast CIN genes and human homologs tested in the one-to-one complementation screen. (.xlsx, 40 KB)
Table S3 - Essential yeast genes and human homologs included in the pool-to-pool complementation screen. (.xlsx, 53 KB)
Table S4 - Comparison of our compiled list of complementation pairs to literature sources. (.xlsx, 16 KB)
Table S5 - Select examples of essential yeast genes from the one-to-one screen that had multiple homologs tested for complementation. (.xlsx, 12 KB)
Table S6 - Tumor-specific variants analyzed in a yeast wild-type background. (.xlsx, 14 KB)