Phylogenetic and Kinetic Characterization of a Suite of Dehydrogenases from a Newly Isolated Bacterium, Strain SG61-1L, That Catalyze the Turnover of Guaiacylglycerol-β-Guaiacyl Ether Stereoisomers

Supplemental material

  • Supplemental file 1 -

    Construction of NADH and MPHPV standard curves, chiral column separation of GGE stereoisomers (Fig. S1), growth of SG61 and SYK-6 on monoaryl compounds over time (Fig. S2), alignment of dehydrogenase genes (Fig. S3), SDS-PAGE gel of purified GGE dehydrogenase enzymes (Fig. S4), retention times and m/z values for metabolites observed during GGE degradation (Table S1), and protein names and corresponding accession numbers for genes encoding proteins included in the phylogenetic analysis (Table S2).

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