Reevaluation of the role of Pex1 and dynamin-related proteins in peroxisome membrane biogenesis

Files in this Data Supplement:

• Supplemental Materials (PDF)
• Video 1 -
  Segregation of de novo–formed peroxisomes in vps1/dnm1 cells. Haploid vps1/dnm1 cells with galactose-controllable Pex19 constitutively expressing HcRed-PTS1 (red) were grown in media containing galactose. Image acquisition started 4.5 h after first exposure to galactose. Images were acquired using time-lapse epifluorescence microscopy on a widefield microscope (Axio Observer). Stacks were taken every 10 min for 15 h. Video runs at one frame per second.
• Video 2 -
  Asymmetric segregation of BiFC complexes with HcRed-PTS1 in vps1 cells. Diploid vps1 cells expressing both Pex13-VN and Pex14-VC (green) and HcRed-PTS1 (red). Images were acquired using time-lapse epifluorescence microscopy on a widefield microscope (Axio Observer). Stacks were taken every 20 min for 14 h. Video runs at one frame per second.