Nano-imaging of the beating mouse heart in vivo: Importance of sarcomere dynamics, as opposed to sarcomere length per se, in the regulation of cardiac function

Files in this Data Supplement:

• Supplemental Materials (PDF)
• Video 1 -
  Ventricular myocyte in the beating heart in vivo (without image sequence reconstruction). Cardiomyocyte expressing α-actinin–AcGFP located in the central part of the LV was imaged (see Fig. 4). Objective lens, 40× (N/A, 0.80; water immersion). Speed, 100 fps. Bar, 10 µm.
• Video 2 -
  Ventricular myocyte in the beating heart in vivo with image sequence reconstruction. Cardiomyocyte expressing α-actinin–AcGFP located in the central part of the LV was imaged (see Figs. 6B and S4, A–D). Objective lens, 60× (N/A, 1.00; water immersion). Speed, 100 fps. Bar, 10 µm.
• Video 3 -
  Ventricular myocyte in the beating heart in vivo without image sequence reconstruction. Cardiomyocyte is the same as in Video 2. Note periodic defocused images as a result of movement of the heart. Objective lens, 60× (N/A, 1.00; water immersion). Speed, 100 fps. Bar, 10 µm.