Fig. S1. C. neoformans is internalized by or associated with neutrophils in the presence of wild-type mouse serum. Fig. S2. Percentages of phagocytosis and killing of 52D and B3501 strains. Fig. S3. ROI production of neutrophils stimulated by C. neoformans is significantly reduced in the presence of C3−/− or C5−/− serum compared to wild-type serum. Fig. S4. Killing of C. neoformans is associated with phagocytosis. Fig. S5. Coincubation of neutrophils with C. neoformans leads to activation of Erk and p38 pathways. Legends for Videos S1 to S8.
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Video S1. A 3D reconstructive image showing that C. neoformans (green) was internalized by neutrophils (red) in the presence of wild-type mouse serum.
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Video S2. Neutrophils chasing yeast cells, followed by internalization of the organisms, in the presence of wild-type mouse serum.
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Video S3. Neutrophil chasing a yeast cell in the presence of wild-type mouse serum.
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Video S4. Neutrophils did not chase the yeast cells in the presence of C3−/− mouse serum.
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Video S5. Neutrophils did not chase the yeast cells in the presence of C5−/− mouse serum.
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Video S6. Neutrophils travelled actively although they did not chase the yeast cells in the presence of C5−/− mouse serum with addition of rC5a.
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Video S7. CD11b−/− neutrophils chasing the yeast cells in the presence of wild-type mouse serum.
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Video S8. Neutrophils pretreated with DIDS chasing the yeast cells, followed by attaching to the yeast cells without further phagocytosis, in the presence of wild-type mouse serum.
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