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Fig. 7. Percentage of global hydrogen exchange at five temperatures. The definition of fast, intermediate, slow, and nonexchanging Hs is described in Results. The percentage of the four groups of Hs for the whole protein at five temperatures is summarized. At lower temperatures (10 and 20°C), the percentage of exchangeable Hs (» 43%), including the fast, intermediate, and slow-exchanging Hs, only changes slightly with temperature; starting from 40°C, the percentage of exchangeable Hs increases steadily from 54% to 75%. It appears there is a temperature threshold somewhere between 20°C and 40°C. Below the threshold, the H/D exchange is almost independent of temperature; whereas above the threshold, the H/D exchange increases steadily with increasing temperature.