Table 1. Pepsin digestion-generated peptides used for analysis in this study
Peptide |
htADH peptide sequence |
Mass |
Charge |
Amide H (NH) |
Elution, min |
IN, % |
BE, % |
1 |
MKAAVVEQFKEPLKIKEVEKPTISYGEVL |
3,309.2 |
4 |
26 |
9.1 |
7 |
22 |
2 |
VRIKACGVCHTDL |
1,416.74 |
2 |
12 |
6.8 |
12 |
25 |
3 |
HAAHGDWPVKPKLPLIPGHEGVG |
2,416.8 |
3 |
18 |
8.3 |
1 |
15 |
4 |
EVGPGVTHLKVGDRVGIPWL |
2,131.32 |
3 |
17 |
10.0 |
9 |
25 |
5 |
YSACGHCDY |
1,020.14 |
2 |
8 |
6.2 |
13 |
25 |
6 |
CLSGQETL |
851.11 |
1 |
7 |
4.9 |
3 |
14 |
7 |
CEHQKNAGYSVDGGYAE |
1,828.8 |
2 |
16 |
6.0 |
7 |
19 |
8 |
YVVKIPDNLSF |
1,296.66 |
2 |
9 |
9.3 |
7 |
23 |
9 |
EEAAPIF |
776.93 |
1 |
5 |
7.1 |
4 |
17 |
10 |
CAGVTTYKALKVTGAKPGEW |
2,080.2 |
3 |
18 |
6.4 |
1 |
13 |
11 |
VAIYGIGGLGHVAVQ |
1,456.08 |
2 |
14 |
8.3 |
3 |
17 |
12 |
YAKAMGI |
753.4 |
1 |
6 |
7.5 |
2 |
15 |
13 |
VAVDIGDEKLEL |
1,303.34 |
2 |
11 |
8.6 |
1 |
16 |
14 |
LVVNPLKEDAAKF |
1,445.78 |
2 |
11 |
8.3 |
8 |
22 |
15 |
MKEKVGGVHAAVVT |
1,429.96 |
2 |
13 |
5.6 |
9 |
20 |
16 |
AVSKPAFQSAYNSIRRGGACVLVGL |
2,568.75 |
3 |
23 |
10.3 |
2 |
19 |
17 |
VGLPPEEMPIPIFDTVL |
1868 |
2 |
12 |
10.7 |
9 |
25 |
18 |
NGIKIIGSIVGTRKDLQEALQ |
2,256.78 |
3 |
20 |
9.1 |
8 |
23 |
19 |
FAAEGKVKTIIEVQPLEKINEV |
2,460.03 |
3 |
20 |
9.4 |
1 |
16 |
20 |
DRMLKGQING |
1,133.34 |
2 |
9 |
4.5 |
8 |
19 |
21 |
RVVLTLEDK |
1,074.34 |
2 |
8 |
5.8 |
7 |
18 |
htADH, thermophilic alcohol dehydrogenase; IN, in-exchange correction; BE, back-exchange correction. For correction calculation, see ref. 1 by Resing et al.
1. Resing, K. A., Hoofnagle, A. N. & Ahn, N. G. (1999) J. Am. Soc. Mass Spectrom. 10, 685-702.