Table 1. Pepsin digestion-generated peptides used for analysis in this study

Peptide

htADH peptide sequence

Mass

Charge

Amide H (NH)

Elution, min

IN, %

BE, %

1

MKAAVVEQFKEPLKIKEVEKPTISYGEVL

3,309.2

4

26

9.1

7

22

2

VRIKACGVCHTDL

1,416.74

2

12

6.8

12

25

3

HAAHGDWPVKPKLPLIPGHEGVG

2,416.8

3

18

8.3

1

15

4

EVGPGVTHLKVGDRVGIPWL

2,131.32

3

17

10.0

9

25

5

YSACGHCDY

1,020.14

2

8

6.2

13

25

6

CLSGQETL

851.11

1

7

4.9

3

14

7

CEHQKNAGYSVDGGYAE

1,828.8

2

16

6.0

7

19

8

YVVKIPDNLSF

1,296.66

2

9

9.3

7

23

9

EEAAPIF

776.93

1

5

7.1

4

17

10

CAGVTTYKALKVTGAKPGEW

2,080.2

3

18

6.4

1

13

11

VAIYGIGGLGHVAVQ

1,456.08

2

14

8.3

3

17

12

YAKAMGI

753.4

1

6

7.5

2

15

13

VAVDIGDEKLEL

1,303.34

2

11

8.6

1

16

14

LVVNPLKEDAAKF

1,445.78

2

11

8.3

8

22

15

MKEKVGGVHAAVVT

1,429.96

2

13

5.6

9

20

16

AVSKPAFQSAYNSIRRGGACVLVGL

2,568.75

3

23

10.3

2

19

17

VGLPPEEMPIPIFDTVL

1868

2

12

10.7

9

25

18

NGIKIIGSIVGTRKDLQEALQ

2,256.78

3

20

9.1

8

23

19

FAAEGKVKTIIEVQPLEKINEV

2,460.03

3

20

9.4

1

16

20

DRMLKGQING

1,133.34

2

9

4.5

8

19

21

RVVLTLEDK

1,074.34

2

8

5.8

7

18

htADH, thermophilic alcohol dehydrogenase; IN, in-exchange correction; BE, back-exchange correction. For correction calculation, see ref. 1 by Resing et al.

1. Resing, K. A., Hoofnagle, A. N. & Ahn, N. G. (1999) J. Am. Soc. Mass Spectrom. 10, 685-702.