Table 7. Dissociation constants for NAD+ from htADH as measured by fluorescence quenching titration
T, ° C |
Kd |
10 |
0.72 ± 0.08 |
25 |
0.79 ± 0.1 |
35 |
0.60 ± 0.1 |
45 |
0.68 ± 0.05 |
55 |
1.28 ± 0.2 |
65 |
1.6 ± 0.3 |