Table 7. Dissociation constants for NAD+ from htADH as measured by fluorescence quenching titration

T, ° C

Kd

10

0.72 ± 0.08

25

0.79 ± 0.1

35

0.60 ± 0.1

45

0.68 ± 0.05

55

1.28 ± 0.2

65

1.6 ± 0.3