|
Johnson et al. 10.1073/pnas.0403250101. |
|
Table 2. Relative mRNA levels of pRB and p107 in Lmna-/- cells |
|||||
|
Cell line |
Rb CT |
HPRT CT |
ΔC T |
2-ΔΔCT |
Normalized Rb in -/- relative to +/+ |
|
Lmna+/+ |
32.80 |
26.26 |
|
|
|
|
|
32.86 |
25.84 |
|
|
|
|
|
32.92 |
26.06 |
|
|
|
|
Average |
32.86 |
26.06 |
6.81 |
0.00 |
1.01 |
|
SD |
0.06 |
0.21 |
0.24 |
0.24 |
0.17 |
|
|
|
|
|
|
|
|
Lmna-/- |
32.33 |
25.48 |
|
|
|
|
|
32.44 |
25.67 |
|
|
|
|
|
32.39 |
25.47 |
|
|
|
|
Average |
32.39 |
25.54 |
6.85 |
0.04 |
0.98 |
|
SD |
0.06 |
0.11 |
0.07 |
0.28 |
0.19 |
|
|
|
|
|
|
|
|
|
P107 CT |
HPRT CT |
ΔC T |
ΔΔC T |
Normalized p107 in -/- relative to +/+ |
|
Lmna+/+ |
28.57 |
26.26 |
|
|
|
|
|
28.45 |
25.84 |
|
|
|
|
|
28.85 |
26.06 |
|
|
|
|
Average |
28.62 |
26.06 |
2.57 |
0.00 |
1.01 |
|
SD |
0.21 |
0.21 |
0.24 |
0.24 |
0.17 |
|
|
|
|
|
|
|
|
Lmna-/- |
27.81 |
25.48 |
|
|
|
|
|
27.61 |
25.51 |
|
|
|
|
|
27.48 |
25.00 |
|
|
|
|
Average |
27.64 |
25.33 |
2.30 |
-0.27 |
1.21 |
|
SD |
0.29 |
0.17 |
0.19 |
0.27 |
0.22 |