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Fig. 6. Acetylated H4K16 and POF localization on the D. busckii male X chromosome. H4K16ac and POF colocalize to a high degree, as shown in the combined POF/H4K16ac panel.

Fig. 7. POF localization to the F element in D. ananassae females (Top). POF and MSL3 binds to the male X in D. malerkotliana. POF and MSL3 colocalizes on the X chromosome, as shown by the combined POF/MSL panel, whereas only POF decorates the F element.

Fig. 8. Sequence alignment of the POF amino acid sequences from D. melanogaster (Dm, GenBank accession no. AJ295236), D. ananassae (Da, GenBank accession no. AY545996), and D. pseudoobscura (Dp, as deduced from genomic sequence, GenBank accession no. AADE01000005).