Experimental testing of a new integrated model of the budding yeast Start transition

Supplemental Materials

This article contains the following supporting material:

  • Supplemental Materials
  • Movie 6 - Movie 6 ? cln3 swi6 GAL1pr-WHI5 raff-gal Phase-contrast movie of cln3Δ swi6Δ cells (JPY1582) carrying a GAL1pr-WHI5 plasmid (pMT3445) grown in raffinose and then switched to galactose. Cells were grown in raffinose for 186 min and then grown in galactose for 712 min.
  • Movie 5 - Movie 5 ? cln3 GAL1pr-WHI5 raff-gal Phase-contrast movie of cln3Δ cells (JPY1812) carrying a GAL1pr-WHI5 plasmid (pMT3445) grown in raffinose and then switched to galactose. Cells were grown in raffinose for 366 min and then grown in galactose for 740 min.
  • Movie 2 - Movie 2 ? GAL1pr-CLN2 raff-gal Phase-contrast movie of wild-type cells (JPY1020) carrying a GAL1pr-CLN2 plasmid (pJP6600) grown in raffinose and then switched to galactose. Cells were grown in raffinose for 281 min and then grown in galactose for 400 min.
  • Movie 3 - Movie 3 ? cln3 mbp1 GAL1pr-BCK2 raff-gal Phase-contrast movie of cln3Δ mbp1Δ cells (JPY1451) carrying a GAL1pr-BCK2 plasmid (pJP6596) grown in raffinose and then switched to galactose. Cells were grown in raffinose for 202 min and then grown in galactose for 306 min.
  • Movie 4 - Movie 4 ? swi6 GAL1pr-WHI5 raff-gal Phase-contrast movie of swi6Δ cells (JPY1054) carrying a GAL1pr-WHI5 plasmid (pMT3445) grown in raffinose and then switched to galactose. Cells were grown in raffinose for 152 min and then grown in galactose for 602 min.
  • Movie 1 - Movie 1 ? wt raff-gal Phase-contrast movie of wild-type cells (JPY1017) carrying an empty BG1805 plasmid (pJP6612) grown in raffinose and then switched to galactose. Cells were grown in raffinose for 176 min and then grown in galactose for 253 min.