Chromatin binding of RCC1 during mitosis is important for its nuclear localization in interphase

Supplemental Materials

This article contains the following supporting material:

  • Supplemental Materials
  • Movie 2 - Movie S2 Live cell imaging of Aid-based RCC1 conditional knockout cells (RCC1 -/-, RCC1tTA, Aid-RCC1) in the absence of IAA. This image is control for Movie S1.
  • Movie 1 - Movie S1 Live cell imaging of Aid-based RCC1 conditional knockout cells (RCC1 -/-, RCC1tTA, Aid-RCC1) expressing histone H2B-RFP (red) and GFP-tubulin (green). Cells were maintained in the presence of 2 μg/ml TET, and fluorescence images were acquired at 3-min intervals in the presence of 500 μM IAA.