This PDF file includes:

• Discussion
• Design of the transmembrane co-chaperonin
• Voltage dependence of the GroEL:αHL-GroES_L interaction
• Additional experimental and procedures
• Fig. S1. GroEL-LLV ATPase activity.
• Fig. S2. LDH refolding assay.
• Fig. S3. Representative EM micrograph of single αHL-GroES_L:GroEL complexes.
• Fig. S4. Nucleotide dependency of SR1 interaction with αHL-GroES_L.
• Fig. S5. Expansions of an SR1-induced current blocked.
• Fig. S6. Release of the binary SR1 current.
• Fig. S7. Ternary SR1 current blockades.
• Table S1. Percentage of GroEL-LLV ATPase activity in the presence of GroES or αHL constructs.
• Table S2. Percentage of GroEL ATPase activity in the presence of GroES, αHL constructs, and/or DOC.
• Table S3. MDH refolding yield catalyzed by GroEL in the presence and absence of GroES, αHL constructs, and/or DOC.
• Table S4. LDH refolding yield catalyzed by GroEL in the presence and absence of GroES, αHL constructs, and/or DOC.

Download PDF