############################################################################################################## ## ClinQC Options file (ClinQCOptions.txt) ## ## Note: This configuration file is used when user has expression data for parent1, parent2 and F1. ## ## This file sets the customizable options for running ClinQC. The options include the paths to the ## database cross reference files, paths to executables, annotation files and other parameters of the pipeline ## ## This file contains example parameter settings, which can be used to run the test data set ## provided with ClinQC. ## For running ClinQC with your own data, the example parameter values have to be modified. ## Please note that only the input value but not the parameter name itself should be changed. ## ## Note: 1) Lines starting with # are comment lines. They do not need to be changed. ## 2) Parameter's name is case sensitive. The parameters can be changed ## by replacing the values after the Ò=Ó sign. ## ############################################################################################################## # Output directory: the name of the directory can be changed. #OUTPUT_DIRECTORY = /ClinQC_1.0/sanger_output OUTPUT_DIRECTORY = test_output/sanger_test # Give the number of processors that should be used in ClinQC pipeline. THREAD = 10 ### Sequencing data quality and trimming parameters Minimum_Base_Quality = 20 Minimum_Read_Length = 50 Maximum_Read_Length = 1000 ### Sanger data quality control and trimming parameters for TTUNER Sanger_Trim_Window_Size = 10 Sanger_Trim_Base_Quality = 20 ################################################################################################################################## # # Provide the experiment information files # ################################################################################################################################## ### Target_File contains Sequencing information. (Mandetory) Target_File = test_data/sanger/scf/test_target_file.txt #Target_File = test_data/sanger/ab1/test_target_file.txt ### Provide the Primer and adapter sequences for trimming #Primer_Adapter_File = test_data/sanger/ab1/adapter_primer_sanger.txt ############################################################################################################## # # Third party software/tool executables # Provide the full path to the third party executables to run the ClinQC pipeline. # # ############################################################################################################## ###### FASTQC executables #FASTQC = ClinQC_1.0/executables/FastQC/fastqc FASTQC = executables/FastQC/fastqc ###### Sanger base calling tool #TTUNER = ClinQC_1.0/executables/tracetuner_3.0.6beta/rel/Linux/ttuner TTUNER = executables/tracetuner_3.0.6beta/rel/Linux_64/ttuner ##### AlienTrimmer is used to trim the additional sequences (Adapters and Primers) and Homopolymers #AlienTrimmer = ClinQC_1.0/executables/AlienTrimmer_0.4.0/src/AlienTrimmer.jar AlienTrimmer = executables/AlienTrimmer_0.4.0/src/AlienTrimmer.jar