Files in this Data Supplement:

• Supplemental Materials (PDF)
• Video 3 -
  P-cadherin expression modifies FA organization and dynamics. Top, inverted confocal images of paxillin-GFP in migrating C2C12 LZRS (left) and C2C12 Pcad cells (right) located at the first multicellular row; 1 frame/5 s for 15 min. Arrows indicate the migration direction. Lines in the first frame indicate nontransfected surrounding cells. Bottom, ratio images generated from the time-lapse shown at top to illustrate FA dynamics, with magenta showing the extension and yellow the loss of FAs. Green represents the FA area maintained during the analyzed period.
• Video 1 -
  P-cadherin expression induces CCM. Phase-contrast images of migrating C2C12 LZRS (left) and C2C12 Pcad cells (right); 1 frame/10 min for 15 h.
• Video 4 -
  Migration of cells on plastic versus collagen-coated polyacrylamide gels. Phase-contrast images of migrating C2C12 LZRS and C2C12 Pcad cells cultured on plastic (left) or collagen-coated polyacrylamide gels (right); 1 frame/45 min for 15 h.
• Video 2 -
  E- or R-cadherin expression does not promote CCM. Phase-contrast images of migrating C2C12 Ecad (left) and C2C12 Rcad (right) cells; 1 frame/10 min for 15 h.
• Video 8 -
  FA dynamics. Left, inverted confocal images of paxillin-GFP in migrating C2C12 Pcad Cdc42 shRNA (top) and C2C12 Pcad β-PIX shRNA (bottom) cells located at the first multicellular row; 1 frame/5 s for 15 min. Arrows indicate the migration direction. Lines in the first frame indicate nontransfected surrounding cells. Right, ratio images generated from the time lapse shown on the left to illustrate FA dynamics, with magenta showing the extension and yellow the loss of FAs. Green represents the FA area maintained during the analyzed period.
• Video 7 -
  P-cadherin expression induction of CCM requires Cdc42 and β-PIX. Phase-contrast images of migrating C2C12 Pcad Cdc42 shRNA (left) and C2C12 Pcad β-PIX shRNA cells (right); 1 frame/10 min for 15 h.
• Video 5 -
  P-cadherin expression increases traction forces at the multicellular leading row. Evolution of Tx of migrating C2C12 LZRS (left) and C2C12 Pcad cells (right) during a 10-h time course; 1 frame/30 min. Maximum Tx of cells moving to the left appear in yellow; maximum Tx of cells moving to the right appear in blue.
• Video 10 -
  Cdc42 or β-PIX inhibition perturbs P-cadherin–dependent traction-force generation of migrating cells. Evolution of Tx of migrating C2C12 Pcad Cdc42 shRNA (left) and C2C12 Pcad β-PIX shRNA (right) cells during a 10-h time course; 1 frame/30 min.
• Video 9 -
  Cdc42 and β-PIX are required for the P-cadherin–dependent increase in intercellular stresses. Monolayer stress component σ xx of migrating C2C12 Pcad Cdc42 shRNA (left) and C2C12 Pcad β-PIX shRNA (right) cells; 1 frame/30 min for 10 h.
• Video 6 -
  P-cadherin expression increases intercellular stresses. Monolayer stress component σ xx of migrating C2C12 LZRS (left) and C2C12 Pcad cells (right); 1 frame/30 min for 10 h.
• Table S1 and DataSets (Zipped Files) -
  Table S1 is provided as an Excel file and shows SILAC MaxQuant and Perseus data. Three datasets are also provided for the MatPIV toolbox used to analyze sequential time frames for velocity and angle measurements, the method for FA detection using Cell Profiler software, and the ImageJ macro to measure FA orientation.