APC binds the Miro/Milton motor complex to stimulate transport of mitochondria to the plasma membrane

Supplemental Materials

This article contains the following supporting material:

  • Supplemental Materials
  • Movie 2 - Supplementary Video 2 Mitochondrial movement in migrating cells transfected with GFP2-Mito and either (1) control or (2) mAPC-red siRNAs. Images were acquired every 7 seconds for a total of 5 mins. The middle planes of z-stacks were acquired from time-lapse live cell imaging, and analysed using MTrackJ, then compressed into AVI files using ImageJ at a rate of 5 frames per second.
  • Movie 1 - Supplementary Video 1 Mitochondrial movement in migrating cells transfected with GFP2-Mito and either (1) control or (2) mAPC-red siRNAs. Images were acquired every 7 seconds for a total of 5 mins. The middle planes of z-stacks were acquired from time-lapse live cell imaging, and analysed using MTrackJ, then compressed into AVI files using ImageJ at a rate of 5 frames per second.