Characterization of Rhodococcus rhodochrous strain PY11; phylogenetic tree of 16S rRNA gene of R. rhodochrous PY11 (Fig. S1); ClustalW2 alignment of the amino acid sequence inferred from the 2HP-inducible mRNA (2HP3) with that of ORF106 from the nicotine biodegradation gene cluster of Arthrobacter nicotinovorans (Fig. S2); homologues of the proteins encoded in 2HP degradation hpo locus, with the homologue search of Hpo proteins performed in the NCBI nonredundant protein database (Table S1) and the UniProtKB/SwissProt database (Table S2) using BLAST; bioconversion of 2HP in E. coli BL21(DE3) expressing HpoBCDF (Fig. S3); bioconversion of 2HP in Rhodococcus sp. strain SQ1 transformed with the pHpoBCDEFG plasmid (Fig. S4); 1H NMR and 13C NMR analyses; HPLC-MS analysis of the reaction products of ω-amidase HpoI (Fig. S5); substrate specificity of HpoBCDF dioxygenase (Table S3).
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