Comprehensive genomic analyses of the OM43 clade including a novel species from Red Sea indicate ecotype differentiation among marine methylotrophs

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    Transmission electron microscopy of OM43 cells from the Red Sea growing at 28 ?C in low-nutrient media (Fig. S1); growth measurements at 28?C of the Red Sea isolate MBRS-H7 in the presence or absence of 100 μM methanol (Fig. S2); maximum-likelihood phylogenetic comparison of 16S rRNA gene versus 23S rRNA gene classification of main methylotrophic bacterial groups (Fig. S3); maximum-likelihood phylogeny of xanthorhodopsin proteins based on the classification by Vollmers et al., including members of the OM43 clade and "Ca. Methylopumilus" strains (Fig. S4); fragment recruitment analysis comparing HTCC2181, KB13, and MBRS-H7 against metagenomes from the Red Sea, East Tropical South Pacific, Hawaii Ocean Time Series, and Bermuda Atlantic Time Series at different seasons and depths (Fig. S5).

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