Dissection of Francisella-Host Cell Interactions in Dictyostelium discoideum

Supplemental material

  • Supplemental file 1 -

    Detailed experimental procedures, nucleotide sequence alignment (Fig. S1), representative dot plots (Fig. S2), replication of F. noatunensis subsp. noatunensis in zebrafish (Fig. S3), ultrastructural analysis (Fig. S4), visualization of the rupture of the phagosomal membrane by electron and fluorescence microscopy (Fig. S5), phagosomal maturation and ubiquitination (Fig. S6), and primers (Table S1).

    PDF, 5.2M

  • Supplemental file 2 -

    Life cell microscopy of Dictyostelium Lifeact-RFP infected with wild-type F. noatunensis subsp. noatunensis expressing GFP at 19 hpi (Movie S1).

    AVI, 4.4M

  • Supplemental file 3 -

    Life cell microscopy of Dictyostelium Lifeact-RFP infected with F. noatunensis subsp. noatunensis ΔiglC expressing GFP at 2 hpi (Movie S2).

    AVI, 6.2M