Genome-Wide Investigation of MicroRNAs and Their Targets in Response to Freezing Stress in Medicago sativa L., Based on High-Throughput Sequencing

Supporting Information for Shu et al., 2016

Files in this Data Supplement:

• Figure S1 - Distribution of miRNA reads in three alfalfa libraries. (.pdf, 94 KB)
• Table S5 - Target genes of miRNAs identified by two degradome sequencing libraries. (.pdf, 79 KB)
• Table S6 - Results of GO terms enrichment analysis using topGO in alfalfa. (.pdf, 82 KB)
• Table S7 - The differentially expression of target genes in alfalfa response to cold and/or freezing stresses. (.pdf, 74 KB)
• Table S8 - Function annotation of miRNAs targets excluding TFs identified by degradome sequencing. (.pdf, 80 KB)
• Figure S2 - Length distribution of alfalfa assembly transcripts. (.pdf, 98 KB)
• Figure S3 - Sequence identity distribution of assembly transcripts in Medicago sativa compared to Medicago truncatula. (.pdf, 105 KB)
• Figure S4 - Distribution of sequencing reads in two degradome sequencing libraries. (.pdf, 90 KB)
• Figure S5 - Class distribution of miRNA targets identified by two degradome sequencing libraries. (.pdf, 93 KB)
• Table S1 - Primers sequences of the reverse transcription and quantitative real-time PCR experiments. (.pdf, 75 KB)
• Table S2 - Conservation of miRNA families across three libraries. (.pdf, 83 KB)
• Table S3 - Novel miRNAs identified in three small RNA sequencing libraries. (.pdf, 77 KB)
• Table S4 - The expressions of miRNA genes identified in three alfalfa libraries. (.pdf, 88 KB)
• File S1 - Degradome sequencing analysis results of cold library by CleaveLand4 software. (.txt, 280 KB)
• File S2 - Degradome sequencing analysis results of freezing library by CleaveLand4 software. (.txt, 224 KB)