Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie 1 - Movie S1. Time-lapse aquisition of si Control HUVECs seeded on cytodex beads to follow cell sprouting initiation and elongation. Phase contrast image acquisition was performed every hour over 14 hours. Scale bar, 50 μm.
- Movie 2 - Movie S2. Time-lapse acquisition of siKif26b HUVECs seeded on cytodex beads. In contrast to control conditions, only environment-sensing filopodia extended from the bead without complete cell extension. Scale bar, 50 μm.
- Movie 3 - Movie S3. Time-lapse acquisition of siControl HUVECs stained for DAPI (blue) along the z-axis in phase contrast illumination and fluorescence. 3D reconstruction of a cell extending toward the matrix, showing filopodia extension and nucleus polarization in the same axis. Image acquisition was performed every hour. Scale bar, 20 μm.
- Movie 4 - Movie S4. Time-lapse acquisition of siKif26b-transfected HUVECs stained for DAPI (blue) along the z-axis in phase contrast illumination and fluorescence. 3D reconstruction of a cell tempting to extend, however the nucleus failed to polarize and re-orient in the filopodia axis. Scale bar, 20 μm.