Files in this Data Supplement:

• Supplemental Materials (PDF)
• Video 2 -
  The symmetric division of a Arl2^T30N-expressing NB. Live whole-mount Arl2^T30N larval brain expressing microtubule-associated G147-GFP was used to analyze the division of NBs. The brain was dissected and loaded into a Lab-TekTM chambered coverglass (Thermo Fisher Scientific) filled with dissecting medium supplemented with 2.5% methyl cellulose (Sigma-Aldrich). The time-lapse images of NB division were acquired every 20 s on a confocal microscope (LSM 710; ZEISS). The video was processed with ImageJ and displayed in 15 frames per second, and the stills of the movie are shown in Fig. 2 G. Time is shown in hours:minutes:seconds. Bar, 5 µm.
• Video 1 -
  The asymmetric division of a wild-type control NB. Live whole-mount wild-type brain expressing microtubule-associated G147-GFP was used to analyze the asymmetric cell division of NBs. The brain was dissected and loaded into a Lab-TekTM chambered coverglass (Thermo Fisher Scientific) filled with dissecting medium supplemented with 2.5% methyl cellulose (Sigma-Aldrich). The time-lapse images of NB division were acquired every 20 s on a confocal microscope (LSM 710; ZEISS). The video was processed with ImageJ and displayed at 15 frames per second, and the stills of the movie are shown in Fig. 2 G. Time is shown in hours:minutes:seconds. Bar, 5 µm.