This PDF file includes:

• Materials and Methods
• Fig. S1. Structure and dynamics of tropoelastin constructs.
• Fig. S2. Association by coacervation of WT and WT+22 tropoelastin solutions.
• Fig. S3. Cross-linking, elastic fiber assembly, and cell attachment of tropoelastin constructs.
• Fig. S4. Comparative mass spectrometry spectra of WT and WT+22 tropoelastin.
• Legends for videos S1 and S2
• References (48–57)

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Other Supplementary Material for this manuscript includes the following:

• Video S1 (.mpg format). The WT elastic network model displays a scissors-like motion between the hinge and foot regions, and a twisting motion in the N-terminal coil region.
• Video S2 (.mpg format). The mutant WT+22 displays dynamics that significantly diverge from the WT.