LOCUS pBAV1K_T5_gfp__a 3621 bp ds-DNA circular 20-OCT-2015 DEFINITION pBAV1K-T5-gfp ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers CDS 90..806 /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRYDFFKSAMPEGYVQERTISFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNG IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALLKDPNEKRDHM VLLEFVTAAGITHGMDELYK*" /label=ORF frame 2 /ApEinfo_fwdcolor=#ff8080 /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 gene 96..803 /gene="GFP (0 - 707)" /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRYDFFKSAMPEGYVQERTISFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNG IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALLKDPNEKRDHM VLLEFVTAAGITHGMDELYK*" /label=GFP (0 - 707) /ApEinfo_fwdcolor=#ffff80 /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(120..148) /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRYDFFKSAMPEGYVQERTISFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNG IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALLKDPNEKRDHM VLLEFVTAAGITHGMDELYK*" /label=GFP_R_primer /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=#80ff80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(164..183) /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRYDFFKSAMPEGYVQERTISFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNG IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALLKDPNEKRDHM VLLEFVTAAGITHGMDELYK*" /label=GFPfusionREV_primer /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=#00ff80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 270..299 /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRYDFFKSAMPEGYVQERTISFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNG IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALLKDPNEKRDHM VLLEFVTAAGITHGMDELYK*" /label=Y66 (EGFP) /ApEinfo_fwdcolor=#80ffff /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 743..763 /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRYDFFKSAMPEGYVQERTISFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNG IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALLKDPNEKRDHM VLLEFVTAAGITHGMDELYK*" /label=GFP_F_primer /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 terminator 884..927 /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRYDFFKSAMPEGYVQERTISFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNG IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALLKDPNEKRDHM VLLEFVTAAGITHGMDELYK*" /label=rrnB_T1_terminator /ApEinfo_fwdcolor=#ff80c0 /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(988..1686) /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMD EKKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEI LDYIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLL DIVDDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRA SYAKVLDAETGEIK*" /label=ORF frame 1 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=#ff80ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 gene complement(2621..3415) /gene="KanR1 (variant)" /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMD EKKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEI LDYIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLL DIVDDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRA SYAKVLDAETGEIK*" /label=KanR1 (variant) /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=#ffff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(2621..3415) /translation="MAKMRISPELKKLIEEYRCVKDTEGMSPAKVYKLVGENEILYLK MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEGY EDEQSPEKTIELYAECIRLFHSIDISDCPYTNSLDGRLAELDYLLNNDLADVDCENWE EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF*" /label=ORF frame 3 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3593..3621 /label=T5 promoter (similar to T5 in NCBI) /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 23..64 /label=Two Lac operator sites + RBS /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3536..3559 /label=iGEM 55 forward sequencing primer /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 877..899 /label=iGEM 56 reverse sequencing primer /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1..21 /label=Biobrick prefix /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 810..830 /label=Biobrick suffix /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 AATTCGCGGC CGCTTCTAGA GGAAATCATA AAAAATTTAT TTGCTTTGTG AGCGGATAAC 61 AATTACTAGA GAAAGAGGAG AAATACTAGA TGCGTAAAGG AGAAGAACTT TTCACTGGAG 121 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG 181 GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG 241 GAAAACTACC TGTTCCATGG CCAACACTTG TCACTACTCT GACGTATGGT GTTCAATGCT 301 TTTCCCGTTA TCCGGATCAT ATGAAACGGT ATGACTTTTT CAAGAGTGCC ATGCCCGAAG 361 GTTATGTACA GGAACGCACT ATATCTTTCA AAGATGACGG GAACTACAAG ACGCGTGCTG 421 AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATCGTATCGA GTTAAAAGGT ATTGATTTTA 481 AAGAAGATGG AAACATTCTC GGACACAAAC TCGAGTACAA CTATAACTCA CACAATGTAT 541 ACATCACGGC AGACAAACAA AAGAATGGAA TCAAAGCTAA CTTCAAAATT CGCCACAACA 601 TTGAAGATGG ATCCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG 661 GCCCTGTCCT TTTACCAGAC AACCATTACC TGTCGACACA ATCTGCCCTT TTGAAAGATC 721 CCAACGAAAA GCGTGACCAC ATGGTCCTTC TTGAGTTTGT AACTGCTGCT GGGATTACAC 781 ATGGCATGGA TGAACTATAC AAATAATAAT ACTAGTAGCG GCCGCTGCAG GCCTCAGGGC 841 CCGATCGATG CCGCCGCTTA ATTAATTAAT CCAGAGGCAT CAAATAAAAC GAAAGGCTCA 901 GTCGAAAGAC TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCCTGAGTAG 961 GACAAATCCG CCGCCCTAGA CCTAGTGTCA TTTTATTTCC CCCGTTTCAG CATCAAGAAC 1021 CTTTGCATAA CTTGCTCTAT ATCCACACTG ATAATTGCCC TCAAACCATA ATCTAAAGGC 1081 GCTAGAGTTT GTTGAAACAA TATCTTTTAC ATCATTCGTA TTTAAAATTC CAAACTCCGC 1141 TCCCCTAAGG CGAATAAAAG CCATTAAATC TTTTGTATTT ACCAAATTAT AGTCATCCAC 1201 TATATCTAAG AGTAAATTCT TCAATTCTCT TTTTTGGCTT TCATCAAGTG TTATATAGCG 1261 GTCAATATCA AAATCATTAA TGTTCAAAAT ATCTTTTTTG TCGTATATAT GTTTATTCTT 1321 AGCAATAGCG TCCTTTGATT CATGAGTCAA ATATTCATAT GAACCTTTGA TATAATCAAG 1381 TATCTCAACA TGAGCAACTG AACTATTCCC CAATTTTCGC TTAATCTTGT TCCTAACGCT 1441 TTCTATTGTT ACAGGATTTC GTGCAATATA TATAACGTGA TAGTGTGGTT TTTTATAGTG 1501 CTTTCCATTT CGTATAACAT CACTACTATT CCATGTATCT TTATCTTTTT TTTCGTCCAT 1561 ATCGTGTAAA GGACTGACAG CCATAGATAC GCCCAAACTC TCTAATTTTT CCTTCCAATC 1621 ATTAGGAATT GAGTCAGGAT ATAATAAAAA TCCAAAATTT CTAGCTTTAG TATTTTTAAT 1681 AGCCATGATA TAATTACCTT ATCAAAAACA AGTAGCGAAA ACTCGTATCC TTCTAAAAAC 1741 GCGAGCTTTC GCTTATTTTT TTTGTTCTGA TTCCTTTCTT GCATATTCTT CTATAGCTAA 1801 CGCCGCAACC GCAGATTTTG AAAAACCTTT TTGTTTCGCC ATATCTGTTA ATTTTTTATC 1861 TTGCTCTTTT GTCAGAGAAA TCATAACTCT TTTTTTCGAT TCTGAAATCA CCATTTAAAA 1921 AACTCCAATC AAATAATTTT ATAAAGTTAG TGTATCACTT TGTAATCATA AAAACAACAA 1981 TAAAGCTACT TAAATATAGA TTTATAAAAA ACGTTGGCGA AAACGTTGGC GATTCGTTGG 2041 CGATTGAAAA ACCCCTTAAA CCCTTGAGCC AGTTGGGATA GAGCGTTTTT GGCACAAAAA 2101 TTGGCACTCG GCACTTAATG GGGGGTCGTA GTACGGAAGC AAAATTCGCT TCCTTTCCCC 2161 CCATTTTTTT CCAAATTCCA AATTTTTTTC AAAAATTTTC CAGCGCTACC GCTCGGCAAA 2221 ATTGCAAGCA ATTTTTAAAA TCAAACCCAT GAGGGAATTT CATTCCCTCA TACTCCCTTG 2281 AGCCTCCTCC AACCGAAATA GAAGGGCGCT GCGCTTATTA TTTCATTCAG TCATCGGCTT 2341 TCATAATCTA ACAGACAACA TCTTCGCTGC AAAGCCACGC TACGCTCAAG GGCTTTTACG 2401 CTACGATAAC GCCTGTTTTA ACGATTATGC CGATAACTAA ACGAAATAAA CGCTAAAACG 2461 TCTCAGAAAC GATTTTGAGA CGTTTTAATA AAAAATCGCC TAGTGCTTGG ATTCTCACCA 2521 ATAAAAAACG CCCGGCGGCA ACCGAGCGTT CTGAACAAAT CCAGATGGAG TTCTGAGGTC 2581 ATTACTGGAT CTATCAACAG GAGTCCAAGC GAGCTCGGTA CTAAAACAAT TCATCCAGTA 2641 AAATATAATA TTTTATTTTC TCCCAATCAG GCTTGATCCC CAGTAAGTCA AAAAATAGCT 2701 CGACATACTG TTCTTCCCCG ATATCCTCCC TGATCGACCG GACGCAGAAG GCAATGTCAT 2761 ACCACTTGTC CGCCCTGCCG CTTCTCCCAA GATCAATAAA GCCACTTACT TTGCCATCTT 2821 TCACAAAGAT GTTGCTGTCT CCCAGGTCGC CGTGGGAAAA GACAAGTTCC TCTTCGGGCT 2881 TTTCCGTCTT TAAAAAATCA TACAGCTCGC GCGGATCTTT AAATGGAGTG TCTTCTTCCC 2941 AGTTTTCGCA ATCCACATCG GCCAGATCGT TATTCAGTAA GTAATCCAAT TCGGCTAAGC 3001 GGCCGTCTAA GCTATTCGTA TAGGGACAAT CCGATATGTC GATGGAGTGA AAGAGCCTGA 3061 TGCACTCCGC ATACAGCTCG ATAGTCTTTT CAGGGCTTTG TTCATCTTCA TACCCTTCCG 3121 AGCAAAGGAC GCCATCGGCC TCACTCATGA GCAGATTGCT CCAGCCATCA TGCCGTTCAA 3181 AGTGCAGGAC CTTTGGAACA GGCAGCTTTC CTTCCAGCCA TAGCATCATG TCCTTTTCCC 3241 GTTCCACATC ATAGGTGGTC CCTTTATACC GGCTGTCCGT CATTTTTAAA TATAGGATTT 3301 CATTTTCTCC CACCAGCTTA TATACCTTAG CAGGAGACAT TCCTTCCGTA TCTTTTACGC 3361 AGCGGTATTC TTCGATCAGT TTTTTCAATT CCGGTGATAT TCTCATTTTA GCCATTTATT 3421 ATTTCCTTCC TCTTTTCTAC AGTATTTAAA GATACCCCAA GAAGCTAATT ATAACAAGAC 3481 GAACTCCAAT TCACTGTTCC TTGCATTCTA AAACCTTAAA TACAGAAAAC AGCCTTTTCA 3541 AAGTTGTTTT CAAAGTTGGC GTATAACATA GTATCGACGG AGCCGATTTT GAAACCACAA 3601 TTATGATAGA ATTTGACGTC G //