LOCUS Supplementary_Da 3797 bp ds-DNA circular 17-FEB-2016 DEFINITION Cloning vector pSEVA331, complete sequence. ACCESSION JX560333 VERSION JX560333.1 GI:410073691 KEYWORDS . SOURCE Cloning vector pSEVA331 ORGANISM Cloning vector pSEVA331 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 2968) AUTHORS Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B., Arce-Rodriguez,A., de las Heras,A., Paez-Espino,D., Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R. and de Lorenzo,V. TITLE The Standard European Vector Architecture (SEVA): a coherent platform for analysis and deployment of complex prokaryotic phenotypes JOURNAL Unpublished REFERENCE 2 (bases 1 to 2968) AUTHORS Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B., Arce-Rodriguez,A., de las Heras,A., Paez-Espino,D., Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R. and de Lorenzo,V. TITLE Direct Submission JOURNAL Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, Madrid 28049, Spain COMMENT ##Assembly-Data-START## Assembly Method :: ApE v. v2.0.40 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers terminator 926..1028 /note="T0" /label=T0 /ApEinfo_fwdcolor=#80ffff /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 913..925 /label=Suffix /ApEinfo_fwdcolor=#8080ff /ApEinfo_revcolor=#8080ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 37..42 /label=DNA: SpeI /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1175..1834 /gene="cat" /codon_start=1 /transl_table=11 /product="chloramphenicol acetyltransferase" /protein_id="AFV59785.1" /db_xref="GI:410073692" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A" /label=chloramphenicol acetyltransferase /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 44..55 /label=BBa_B0034 RBS /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1858..2093 /note="oriT" /label=oriT /ApEinfo_fwdcolor=#80ff00 /ApEinfo_revcolor=#80ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 RBS 44..55 /label=RBS /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 914..920 /label=NotI /ApEinfo_fwdcolor=#0080c0 /ApEinfo_revcolor=#0080c0 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(2102..2764) /gene="rep" /codon_start=1 /transl_table=11 /product="replication protein" /protein_id="AFV59786.1" /db_xref="GI:410073693" /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP RR" /label=replication protein /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=#004080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 920..925 /label=PstI /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 62..742 /gene="mRFP" /label=mRFP /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(2501..2656) /gene="rep" /note="Helix-turn-helix domain; Region: HTH_36; pfam13730" /db_xref="CDD:258026" /label=rep /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=#c0c0c0 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 776..904 /label=BBa_B0015 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(945..968) /label=Reverse primer /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=#0080ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 stem_loop 787..830 /label=rrnB terminator /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2095..2100 /label=NgoMIV Re site /ApEinfo_fwdcolor=#cd0532 /ApEinfo_revcolor=#cd0532 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3776..3797 /label=BioBrick prefix and suffix /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 3752..3775 /note="F24" /label=F24 /ApEinfo_fwdcolor=#ffff80 /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 terminator 3638..3742 /note="T1" /label=T1 /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3641..3658 /label=Forward sequencing primer /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1..35 /label=BBa_J23100 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gtgaaagagg agaaatacta 61 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg 121 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg 181 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat 241 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc 301 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga 361 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta 421 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac 481 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga 541 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac 601 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga 661 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg 721 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg 781 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 841 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 901 tatatactag aagcggccgc tgcagcttgg actcctgttg atagatccag taatgacctc 961 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga 1021 gaatccaggg gtccccaata attacgattt aaattggcga aaatgagacg ttgatcggca 1081 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga 1141 gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac 1201 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc 1261 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa 1321 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc 1381 tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca 1441 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata 1501 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga 1561 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc 1621 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc 1681 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat 1741 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca 1801 acagtactgc gatgagtggc agggcggggc gtaatttgac ttttgtcctt ttccgctgca 1861 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga 1921 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag 1981 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct 2041 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaggccggc 2101 cctaccggcg cggcagcgtt acccgtgtcg gcggctccaa cggctcgcca tcgtccagaa 2161 aacacggctc atcgggcatc ggcaggcgct gctgcccgcg ccgttcccat tcctccgttt 2221 cggtcaaggc tggcaggtct ggttccatgc ccggaatgcc gggctggctg ggcggctcct 2281 cgccggggcc ggtcggtagt tgctgctcgc ccggatacag ggtcgggatg cggcgcaggt 2341 cgccatgccc caacagcgat tcgtcctggt cgtcgtgatc aaccaccacg gcggcactga 2401 acaccgacag gcgcaactgg tcgcggggct ggccccacgc cacgcggtca ttgaccacgt 2461 aggccgacac ggtgccgggg ccgttgagct tcacgacgga gatccagcgc tcggccacca 2521 agtccttgac tgcgtattgg accgtccgca aagaacgtcc gatgagcttg gaaagtgtct 2581 tctggctgac caccacggcg ttctggtggc ccatctgcgc cacgaggtga tgcagcagca 2641 ttgccgccgt gggtttcctc gcaataagcc cggcccacgc ctcatgcgct ttgcgttccg 2701 tttgcaccca gtgaccgggc ttgttcttgg cttgaatgcc gatttctctg gactgcgtgg 2761 ccatgcttat ctccatgcgg taggggtgcc gcacggttgc ggcaccatgc gcaatcagct 2821 gcaacttttc ggcagcgcga caacaattat gcgttgcgta aaagtggcag tcaattacag 2881 attttcttta acctacgcaa tgagctattg cggggggtgc cgcaatgagc tgttgcgtac 2941 cccccttttt taagttgttg atttttaagt ctttcgcatt tcgccctata tctagttctt 3001 tggtgcccaa agaagggcac ccctgcgggg ttcccccacg ccttcggcgc ggctccccct 3061 ccggcaaaaa gtggcccctc cggggcttgt tgatcgactg cgcggccttc ggccttgccc 3121 aaggtggcgc tgcccccttg gaacccccgc actcgccgcc gtgaggctcg gggggcaggc 3181 gggcgggctt cgcccttcga ctgcccccac tcgcataggc ttgggtcgtt ccaggcgcgt 3241 caaggccaag ccgctgcgcg gtcgctgcgc gagccttgac ccgccttcca cttggtgtcc 3301 aaccggcaag cgaagcgcgc aggccgcagg ccggaggctt ttccccagag aaaattaaaa 3361 aaattgatgg ggcaaggccg caggccgcgc agttggagcc ggtgggtatg tggtcgaagg 3421 ctgggtagcc ggtgggcaat ccctgtggtc aagctcgtgg gcaggcgcag cctgtccatc 3481 agcttgtcca gcagggttgt ccacgggccg agcgaagcga gccagccggt ggccgctcgc 3541 ggccatcgtc cacatatcca cgggctggca agggagcgca gcgaccgcgc agggcgaagc 3601 ccggagagca agcccgtagg gggggcgcgc ccagctgtct agggcggcgg atttgtccta 3661 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga 3721 gcctttcgtt ttatttgatg cctttaatta aagcggataa caatttcaca caggaGAATT 3781 CGCGGCCGCT TCTAGAG //