Fig. S1. Levels of Coxiella burnetii Nine Mile II bacterial uptake are comparable in C57BL/6 macrophages and macrophages deficient in TLR signaling components. Fig. S2. Purified Coxiella burnetii Nine Mile II LPS does not elicit cytokine production from C57BL/6 macrophages. Fig. S3. Coxiella burnetii Nine Mile II translocates bacterial effectors into C57BL/6 macrophages in a T4SS-dependent manner. Fig. S4. Coxiella burnetii Nine Mile II does not induce inflammasome activation in permissive TLR2-deficient macrophages, and WT and caspase-1- and caspase-11-deficient macrophages display similar levels of bacterial uptake. Fig. S5. Levels of bacterial uptake are comparable in C57BL/6 and TNF-deficient macrophages. Fig. S6. Induction of iNOS and nitric oxide production is decreased in TNF-deficient macrophages following Coxiella burnetii Nine Mile II infection. Fig. S7. Levels of large-vacuole formation are comparable in TLR2-deficient and TNF-deficient macrophages.
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